MSDS Number: S0240 * * * * * Effective Date: 11/21/08 * * * * * Supercedes: 02/13/06 SAFRANINE O


1. Product Identification

Synonyms: CI 50240; basic red 2
CAS No.: 477-73-6
Molecular Weight: 350.85
Chemical Formula: C20H19N4Cl
Product Codes:
J.T. Baker: U926
Mallinckrodt: E008, E057

2. Composition/Information on Ingredients

  Ingredient                                CAS No         Percent        Hazardous                                  
  ---------------------------------------   ------------   ------------   ---------   
  Safranine O                               477-73-6         90 - 100%       Yes                                                                    

3. Hazards Identification

Emergency Overview

SAF-T-DATA(tm) Ratings (Provided here for your convenience)
Health Rating: 1 - Slight
Flammability Rating: 0 - None
Reactivity Rating: 0 - None
Contact Rating: 2 - Moderate
Storage Color Code: Green (General Storage)

Potential Health Effects

Information on the human health effects from exposure to this substance is limited.

No information found, but compound should be handled as a potential health hazard.
No information found, but compound should be handled as a potential health hazard.
Skin Contact:
Causes irritation to skin. Symptoms include redness, itching, and pain.
Eye Contact:
Causes irritation, redness, and pain.
Chronic Exposure:
No information found.
Aggravation of Pre-existing Conditions:
No information found.

4. First Aid Measures

Remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical attention.
Induce vomiting immediately as directed by medical personnel. Never give anything by mouth to an unconscious person. Get medical attention.
Skin Contact:
Immediately flush skin with plenty of water for at least 15 minutes. Remove contaminated clothing and shoes. Get medical attention. Wash clothing before reuse. Thoroughly clean shoes before reuse.
Eye Contact:
Immediately flush eyes with plenty of water for at least 15 minutes, lifting lower and upper eyelids occasionally. Get medical attention immediately.

5. Fire Fighting Measures

Not considered to be a fire hazard.
Not considered to be an explosion hazard.
Fire Extinguishing Media:
Use any means suitable for extinguishing surrounding fire.
Special Information:
In the event of a fire, wear full protective clothing and NIOSH-approved self-contained breathing apparatus with full facepiece operated in the pressure demand or other positive pressure mode.

6. Accidental Release Measures

Ventilate area of leak or spill. Wear appropriate personal protective equipment as specified in Section 8. Spills: Sweep up and containerize for reclamation or disposal. Vacuuming or wet sweeping may be used to avoid dust dispersal.

7. Handling and Storage

Keep in a tightly closed container, stored in a cool, dry, ventilated area. Protect against physical damage. Containers of this material may be hazardous when empty since they retain product residues (dust, solids); observe all warnings and precautions listed for the product.

8. Exposure Controls/Personal Protection

Airborne Exposure Limits:
None established.
Ventilation System:
A system of local and/or general exhaust is recommended to keep employee exposures as low as possible. Local exhaust ventilation is generally preferred because it can control the emissions of the contaminant at its source, preventing dispersion of it into the general work area. Please refer to the ACGIH document, Industrial Ventilation, A Manual of Recommended Practices, most recent edition, for details.
Personal Respirators (NIOSH Approved):
For conditions of use where exposure to dust or mist is apparent and engineering controls are not feasible, a particulate respirator (NIOSH type N95 or better filters) may be worn. If oil particles (e.g. lubricants, cutting fluids, glycerine, etc.) are present, use a NIOSH type R or P filter. For emergencies or instances where the exposure levels are not known, use a full-face positive-pressure, air-supplied respirator. WARNING: Air-purifying respirators do not protect workers in oxygen-deficient atmospheres.
Skin Protection:
Wear protective gloves and clean body-covering clothing.
Eye Protection:
Use chemical safety goggles. Maintain eye wash fountain and quick-drench facilities in work area.

9. Physical and Chemical Properties

Dark rusty crystals or dark green powder.
Soluble in water.
Specific Gravity:
No information found.
No information found.
% Volatiles by volume @ 21C (70F):
Boiling Point:
No information found.
Melting Point:
No information found.
Vapor Density (Air=1):
No information found.
Vapor Pressure (mm Hg):
No information found.
Evaporation Rate (BuAc=1):
No information found.

10. Stability and Reactivity

Stable under ordinary conditions of use and storage.
Hazardous Decomposition Products:
May produce carbon monoxide, carbon dioxide, nitrogen oxides and hydrogen chloride when heated to decomposition.
Hazardous Polymerization:
Will not occur.
No information found.
Conditions to Avoid:
No information found.

11. Toxicological Information

No LD50/LC50 information found relating to normal routes of occupational exposure.

Investigated as a mutagen.
  --------\Cancer Lists\------------------------------------------------------
                                         ---NTP Carcinogen---
  Ingredient                             Known    Anticipated    IARC Category
  ------------------------------------   -----    -----------    -------------
  Safranine O (477-73-6)                  No          No            None

12. Ecological Information

Environmental Fate:
No information found.
Environmental Toxicity:
No information found.

13. Disposal Considerations

Whatever cannot be saved for recovery or recycling should be managed in an appropriate and approved waste disposal facility. Processing, use or contamination of this product may change the waste management options. State and local disposal regulations may differ from federal disposal regulations. Dispose of container and unused contents in accordance with federal, state and local requirements.

14. Transport Information

Not regulated.

15. Regulatory Information

  --------\Chemical Inventory Status - Part 1\---------------------------------
  Ingredient                                       TSCA  EC   Japan  Australia
  -----------------------------------------------  ----  ---  -----  ---------
  Safranine O (477-73-6)                            Yes  Yes   Yes      Yes                                      
  --------\Chemical Inventory Status - Part 2\---------------------------------
  Ingredient                                       Korea  DSL   NDSL  Phil.
  -----------------------------------------------  -----  ---   ----  -----
  Safranine O (477-73-6)                            Yes   Yes   No     Yes   
  --------\Federal, State & International Regulations - Part 1\----------------
                                             -SARA 302-    ------SARA 313------
  Ingredient                                 RQ    TPQ     List  Chemical Catg.
  -----------------------------------------  ---   -----   ----  --------------
  Safranine O (477-73-6)                     No    No      No         No
  --------\Federal, State & International Regulations - Part 2\----------------
                                                        -RCRA-    -TSCA-
  Ingredient                                 CERCLA     261.33     8(d) 
  -----------------------------------------  ------     ------    ------
  Safranine O (477-73-6)                     No         No         No 
Chemical Weapons Convention:  No     TSCA 12(b):  No     CDTA:  No
SARA 311/312:  Acute: Yes      Chronic: No   Fire: No  Pressure: No
Reactivity: No          (Pure / Solid) 

Australian Hazchem Code: None allocated.
Poison Schedule: None allocated.
This MSDS has been prepared according to the hazard criteria of the Controlled Products Regulations (CPR) and the MSDS contains all of the information required by the CPR.

16. Other Information

NFPA Ratings: Health: 1 Flammability: 0 Reactivity: 0
Label Hazard Warning:
Label Precautions:
Avoid contact with eyes, skin and clothing.
Wash thoroughly after handling.
Label First Aid:
In case of contact, immediately flush eyes or skin with plenty of water for at least 15 minutes. Remove contaminated clothing and shoes. Wash clothing before reuse. Get medical attention.
Product Use:
Laboratory Reagent.
Revision Information:
No Changes.
Prepared by: Environmental Health & Safety
Phone Number: (314) 654-1600 (U.S.A.)