MSDS Number: C4807 * * * * * Effective Date: 02/15/08 * * * * * Supercedes: 04/28/05 DITHIOTHREITOL


DITHIOTHREITOL


1. Product Identification

Synonyms: Threitol, 1,4-dithio-,dl-; 2,3-butanediol, 1,4-dimercapto dl-threo; Cleland's Reagent; DTT
CAS No.: 3483-12-3 (also identified as CAS# 27565-41-9)
Molecular Weight: 154.26
Chemical Formula: C4H10O2S2
Product Codes:
J.T. Baker: F780, F781
Mallinckrodt: 7750

2. Composition/Information on Ingredients


 
  Ingredient                                CAS No         Percent        Hazardous                                  
  ---------------------------------------   ------------   ------------   ---------   
 
  1,4-Dithiothreitol (Clelands Reagent)     3483-12-3          100%          Yes                                                                 
 

3. Hazards Identification

Emergency Overview
--------------------------
WARNING! HARMFUL IF SWALLOWED OR INHALED. MAY BE HARMFUL IF ABSORBED THROUGH SKIN. CAUSES IRRITATION TO SKIN, EYES AND RESPIRATORY TRACT. MAY AFFECT CENTRAL NERVOUS SYSTEM.

SAF-T-DATA(tm) Ratings (Provided here for your convenience)
-----------------------------------------------------------------------------------------------------------
Health Rating: 2 - Moderate
Flammability Rating: 1 - Slight
Reactivity Rating: 0 - None
Contact Rating: 2 - Moderate
Lab Protective Equip: GOGGLES; LAB COAT; VENT HOOD; PROPER GLOVES
Storage Color Code: Green (General Storage)
-----------------------------------------------------------------------------------------------------------

Potential Health Effects
----------------------------------

Inhalation:
Causes irritation to the respiratory tract. Symptoms may include coughing, shortness of breath. Can cause nausea, headache and vomiting. Exposure may result in blood in urine, difficulty breathing, irregular heartbeat, anaemia, weakness, drunkenness, bluish skin color, lung congestion, kidney damage, paralysis, convulsions unconsciousness and coma. Thiols may cause central nervous system depression (CNS).
Ingestion:
Exposure can cause nausea, headache, vomiting, diarrhea, weakness, drunkenness, restlessness, bluish skin color, paralysis and coma.
Skin Contact:
Causes irritation to skin. Symptoms include redness, itching, and pain. May be absorbed through the skin. May cause dermatitis.
Eye Contact:
Causes irritation, redness, and pain.
Chronic Exposure:
No information found.
Aggravation of Pre-existing Conditions:
No information found.

4. First Aid Measures

Inhalation:
Remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical attention.
Ingestion:
Give large amounts of water to drink. Never give anything by mouth to an unconscious person. Get medical attention.
Skin Contact:
Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Wash clothing before reuse. Thoroughly clean shoes before reuse. Get medical attention if symptoms occur.
Eye Contact:
Immediately flush eyes with plenty of water for at least 15 minutes, lifting upper and lower eyelids occasionally. Get medical attention if irritation persists.

5. Fire Fighting Measures

Fire:
Flash point: > 109C (> 228F) CC
As with most organic solids, fire is possible at elevated temperatures or by contact with an ignition source.
Explosion:
Fine dust dispersed in air in sufficient concentrations, and in the presence of an ignition source is a potential dust explosion hazard.
Fire Extinguishing Media:
Water spray, dry chemical, alcohol foam, or carbon dioxide.
Special Information:
In the event of a fire, wear full protective clothing and NIOSH-approved self-contained breathing apparatus with full facepiece operated in the pressure demand or other positive pressure mode.

6. Accidental Release Measures

Ventilate area of leak or spill. Wear appropriate personal protective equipment as specified in Section 8. Spills: Pick up and place in a suitable container for reclamation or disposal, using a method that does not generate dust.



7. Handling and Storage

Product must be refrigerated at 2 - 8C (36 - 46F). Keep in a tightly closed container. Protect against physical damage. Handle and store under nitrogen. Containers of this material may be hazardous when empty since they retain product residues (dust, solids); observe all warnings and precautions listed for the product.

8. Exposure Controls/Personal Protection

Airborne Exposure Limits:
None established.
Ventilation System:
A system of local and/or general exhaust is recommended to keep employee exposures as low as possible. Local exhaust ventilation is generally preferred because it can control the emissions of the contaminant at its source, preventing dispersion of it into the general work area. Please refer to the ACGIH document, Industrial Ventilation, A Manual of Recommended Practices, most recent edition, for details.
Personal Respirators (NIOSH Approved):
For conditions of use where exposure to the dust or mist is apparent, a half-face dust/mist respirator may be worn. For emergencies or instances where the exposure levels are not known, use a full-face positive-pressure, air-supplied respirator. WARNING: Air-purifying respirators do not protect workers in oxygen-deficient atmospheres.
Skin Protection:
Wear protective gloves and clean body-covering clothing.
Eye Protection:
Use chemical safety goggles. Maintain eye wash fountain and quick-drench facilities in work area.

9. Physical and Chemical Properties

Appearance:
White crystals.
Odor:
Thiol odor.
Solubility:
Soluble in water.
Specific Gravity:
1.0
pH:
4.3 (5% solution)
% Volatiles by volume @ 21C (70F):
0
Boiling Point:
125 - 130C (257 - 266F)
Melting Point:
42 - 43C (108 - 109F)
Vapor Density (Air=1):
5.3
Vapor Pressure (mm Hg):
No information found.
Evaporation Rate (BuAc=1):
No information found.

10. Stability and Reactivity

Stability:
Stable under ordinary conditions of use and storage. Hygroscopic.
Hazardous Decomposition Products:
Burning may produce carbon monoxide, carbon dioxide, sulfur oxides.
Hazardous Polymerization:
Will not occur.
Incompatibilities:
Strong oxidizers.
Conditions to Avoid:
Heat, flames, ignition sources and incompatibles.

11. Toxicological Information


For 1,4-Dithiothreitol: Oral rat LD50: 400 mg/kg. Investigated as a mutagen.
 
  --------\Cancer Lists\------------------------------------------------------
                                         ---NTP Carcinogen---
  Ingredient                             Known    Anticipated    IARC Category
  ------------------------------------   -----    -----------    -------------
  1,4-Dithiothreitol (Clelands            No          No            None
  Reagent) (3483-12-3)

12. Ecological Information

Environmental Fate:
No information found.
Environmental Toxicity:
No information found.

13. Disposal Considerations

Whatever cannot be saved for recovery or recycling should be managed in an appropriate and approved waste disposal facility. Processing, use or contamination of this product may change the waste management options. State and local disposal regulations may differ from federal disposal regulations. Dispose of container and unused contents in accordance with federal, state and local requirements.

14. Transport Information

Not regulated.

15. Regulatory Information

 
  --------\Chemical Inventory Status - Part 1\---------------------------------
  Ingredient                                       TSCA  EC   Japan  Australia
  -----------------------------------------------  ----  ---  -----  ---------
  1,4-Dithiothreitol (Clelands Reagent)             Yes  Yes   No       Yes                                      
  (3483-12-3)
 
  --------\Chemical Inventory Status - Part 2\---------------------------------
                                                          --Canada--
  Ingredient                                       Korea  DSL   NDSL  Phil.
  -----------------------------------------------  -----  ---   ----  -----
  1,4-Dithiothreitol (Clelands Reagent)             No    Yes   No     No                    
  (3483-12-3)
 
  --------\Federal, State & International Regulations - Part 1\----------------
                                             -SARA 302-    ------SARA 313------
  Ingredient                                 RQ    TPQ     List  Chemical Catg.
  -----------------------------------------  ---   -----   ----  --------------
  1,4-Dithiothreitol (Clelands Reagent)      No    No      No         No
  (3483-12-3)
 
  --------\Federal, State & International Regulations - Part 2\----------------
                                                        -RCRA-    -TSCA-
  Ingredient                                 CERCLA     261.33     8(d) 
  -----------------------------------------  ------     ------    ------
  1,4-Dithiothreitol (Clelands Reagent)      No         No         No                 
  (3483-12-3)
 
 
Chemical Weapons Convention:  No     TSCA 12(b):  No     CDTA:  No
SARA 311/312:  Acute: Yes      Chronic: No   Fire: No  Pressure: No
Reactivity: No          (Pure / Solid) 


Australian Hazchem Code: None allocated.
Poison Schedule: None allocated.
WHMIS:
This MSDS has been prepared according to the hazard criteria of the Controlled Products Regulations (CPR) and the MSDS contains all of the information required by the CPR.

16. Other Information

NFPA Ratings: Health: 2 Flammability: 1 Reactivity: 0
Label Hazard Warning:
WARNING! HARMFUL IF SWALLOWED OR INHALED. MAY BE HARMFUL IF ABSORBED THROUGH SKIN. CAUSES IRRITATION TO SKIN, EYES AND RESPIRATORY TRACT. MAY AFFECT CENTRAL NERVOUS SYSTEM.
Label Precautions:
Avoid breathing dust.
Avoid contact with eyes, skin and clothing.
Keep container closed.
Use with adequate ventilation.
Wash thoroughly after handling.
Store refrigerated.
Handle and store under nitrogen.
Label First Aid:
If swallowed, give large amounts of water to drink. Never give anything by mouth to an unconscious person. If inhaled, remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. In case of contact, immediately flush eyes or skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Wash clothing before reuse. In all cases, get medical attention.
Product Use:
Laboratory Reagent.
Revision Information:
No Changes.
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Prepared by: Environmental Health & Safety
Phone Number: (314) 654-1600 (U.S.A.)