Synonyms: Homidium bromide; dromilac; 3,8-diamino-5-ethyl-6-phenyl phenanthridinium bromide
CAS No.: 1239-45-8
Molecular Weight: 394.31
Chemical Formula: C21H20BrN3
Product Codes: 4007
Ingredient CAS No Percent Hazardous --------------------------------------- ------------ ------------ --------- Ethidium Bromide 1239-45-8 90 - 100% Yes
Emergency Overview
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WARNING! MAY BE FATAL IF INHALED. HARMFUL IF SWALLOWED OR ABSORBED THROUGH SKIN. CAUSES IRRITATION TO SKIN, EYES AND RESPIRATORY TRACT. MAY CAUSE HERITABLE GENETIC DAMAGE.
SAF-T-DATA(tm) Ratings (Provided here for your convenience)
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Health Rating: 4 - Extreme (Poison)
Flammability Rating: 1 - Slight
Reactivity Rating: 1 - Slight
Contact Rating: 3 - Severe (Life)
Lab Protective Equip: GOGGLES; LAB COAT; VENT HOOD; PROPER GLOVES
Storage Color Code: Blue (Health)
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Potential Health Effects
----------------------------------
Inhalation:
The dust is very toxic by inhalation. Inhalation of dust irritates the respiratory tract.
Ingestion:
No information found, but compound should be handled as a potential health hazard.
Skin Contact:
Inflammation and discoloration of the skin may occur after contact. Contact will stain the skin purple.
Eye Contact:
Causes irritation, redness, and pain.
Chronic Exposure:
May cause heritable genetic damage.
Aggravation of Pre-existing Conditions:
No information found.
Inhalation:
If inhaled, remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. CALL A PHYSICIAN IMMEDIATELY.
Ingestion:
Induce vomiting immediately as directed by medical personnel. Never give anything by mouth to an unconscious person. Get medical attention.
Skin Contact:
Immediately flush skin with plenty of water for at least 15 minutes. Remove contaminated clothing and shoes. Wash clothing before reuse. Thoroughly clean shoes before reuse. Get medical attention if irritation develops.
Eye Contact:
Immediately flush eyes with plenty of water for at least 15 minutes, lifting upper and lower eyelids occasionally. Get medical attention if irritation persists.
Fire:
As with most organic solids, fire is possible at elevated temperatures or by contact with an ignition source.
Explosion:
Not an explosion hazard under normal conditions of storage and use, but fine dust dispersed in air in sufficient concentrations, and in the presence of an ignition source is a potential dust explosion hazard. Sensitive to static discharge.
Fire Extinguishing Media:
Water spray, dry chemical, alcohol foam, or carbon dioxide.
Special Information:
In the event of a fire, wear full protective clothing and NIOSH-approved self-contained breathing apparatus with full facepiece operated in the pressure demand or other positive pressure mode.
Remove all sources of ignition. Ventilate area of leak or spill. Wear appropriate personal protective equipment as specified in Section 8. Spills: Clean up spills in a manner that does not disperse dust into the air. Use non-sparking tools and equipment. Reduce airborne dust and prevent scattering by moistening with water. Pick up spill for recovery or disposal and place in a closed container.
Avoid dust formation and control ignition sources. Employ grounding, venting and explosion relief provisions in accord with accepted engineering practices in any process capable of generating dust and/or static electricity. Empty only into inert or non-flammable atmosphere. Emptying contents into a non-inert atmosphere where flammable vapors may be present could cause a flash fire or explosion due to electrostatic discharge. Contact will stain the skin purple. Wash gloves before removing them from the hands. Containers of this material may be hazardous when empty since they retain product residues (dust, solids); observe all warnings and precautions listed for the product.
Airborne Exposure Limits:
None established.
Ventilation System:
A system of local and/or general exhaust is recommended to keep employee exposures as low as possible. Local exhaust ventilation is generally preferred because it can control the emissions of the contaminant at its source, preventing dispersion of it into the general work area. Please refer to the ACGIH document, Industrial Ventilation, A Manual of Recommended Practices, most recent edition, for details.
Personal Respirators (NIOSH Approved):
For conditions of use where exposure to dust or mist is apparent and engineering controls are not feasible, a particulate respirator (NIOSH type N95 or better filters) may be worn. If oil particles (e.g. lubricants, cutting fluids, glycerine, etc.) are present, use a NIOSH type R or P filter. For emergencies or instances where the exposure levels are not known, use a full-face positive-pressure, air-supplied respirator. WARNING: Air-purifying respirators do not protect workers in oxygen-deficient atmospheres.
Skin Protection:
Wear protective gloves and clean body-covering clothing.
Eye Protection:
Use chemical safety goggles. Maintain eye wash fountain and quick-drench facilities in work area.
Appearance:
Dark red crystals.
Odor:
Faint characteristic odor.
Solubility:
5 grams in 100 grams of water.
Specific Gravity:
No information found.
pH:
4 - 7 (2% solution)
% Volatiles by volume @ 21C (70F):
No information found.
Boiling Point:
Not applicable.
Melting Point:
238 - 240C (460 - 464F)
Vapor Density (Air=1):
No information found.
Vapor Pressure (mm Hg):
No information found.
Evaporation Rate (BuAc=1):
No information found.
Stability:
Stable under ordinary conditions of use and storage.
Hazardous Decomposition Products:
Toxic gases and vapors may be released if involved in a fire. Oxides of nitrogen, oxides of carbon, hydrogen bromide.
Hazardous Polymerization:
Will not occur.
Incompatibilities:
Strong oxidizers.
Conditions to Avoid:
Heat, flames, ignition sources and incompatibles.
Ethidium bromide: Oral rat LD50: 1503 mg/kg (males); inhalation rat LC50: 0.118 to 0.134 mg/liter. Ethidium bromide is genotoxic in a number of test systems, including Ames, cell mutation, cell transformation and cytogenetics assays.
--------\Cancer Lists\------------------------------------------------------
---NTP Carcinogen---
Ingredient Known Anticipated IARC Category
------------------------------------ ----- ----------- -------------
Ethidium Bromide (1239-45-8) No No None
Environmental Fate:
No information found.
Environmental Toxicity:
No information found.
Whatever cannot be saved for recovery or recycling should be managed in an appropriate and approved waste disposal facility. Processing, use or contamination of this product may change the waste management options. State and local disposal regulations may differ from federal disposal regulations. Dispose of container and unused contents in accordance with federal, state and local requirements.
Domestic (Land, D.O.T.)
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Proper Shipping Name: TOXIC SOLID, ORGANIC, N.O.S.(ETHIDIUM BROMIDE)
Hazard Class: 6.1
UN/NA: UN2811
Packing Group: I
Information reported for product/size: 1G
International (Water, I.M.O.)
-----------------------------
Proper Shipping Name: TOXIC SOLID, ORGANIC, N.O.S.(ETHIDIUM BROMIDE)
Hazard Class: 6.1
UN/NA: UN2811
Packing Group: I
Information reported for product/size: 1G
International (Air, I.C.A.O.)
-----------------------------
Proper Shipping Name: TOXIC SOLID, ORGANIC, N.O.S.(ETHIDIUM BROMIDE)
Hazard Class: 6.1
UN/NA: UN2811
Packing Group: I
Information reported for product/size: 1G
--------\Chemical Inventory Status - Part 1\---------------------------------
Ingredient TSCA EC Japan Australia
----------------------------------------------- ---- --- ----- ---------
Ethidium Bromide (1239-45-8) Exempt Yes No Yes
--------\Chemical Inventory Status - Part 2\---------------------------------
--Canada--
Ingredient Korea DSL NDSL Phil.
----------------------------------------------- ----- --- ---- -----
Ethidium Bromide (1239-45-8) Yes No No Yes
--------\Federal, State & International Regulations - Part 1\----------------
-SARA 302- ------SARA 313------
Ingredient RQ TPQ List Chemical Catg.
----------------------------------------- --- ----- ---- --------------
Ethidium Bromide (1239-45-8) No No No No
--------\Federal, State & International Regulations - Part 2\----------------
-RCRA- -TSCA-
Ingredient CERCLA 261.33 8(d)
----------------------------------------- ------ ------ ------
Ethidium Bromide (1239-45-8) No No No
Chemical Weapons Convention: No TSCA 12(b): No CDTA: No
SARA 311/312: Acute: Yes Chronic: Yes Fire: No Pressure: No
Reactivity: No (Pure / Solid)
Australian Hazchem Code: 2XE
Poison Schedule: None allocated.
WHMIS:
This MSDS has been prepared according to the hazard criteria of the Controlled Products Regulations (CPR) and the MSDS contains all of the information required by the CPR.
NFPA Ratings: Health: 4 Flammability: 1 Reactivity: 0
Label Hazard Warning:
WARNING! MAY BE FATAL IF INHALED. HARMFUL IF SWALLOWED OR ABSORBED THROUGH SKIN. CAUSES IRRITATION TO SKIN, EYES AND RESPIRATORY TRACT. MAY CAUSE HERITABLE GENETIC DAMAGE.
Label Precautions:
Do not breathe dust.
Avoid contact with eyes, skin and clothing.
Keep container closed.
Use with adequate ventilation.
Wash thoroughly after handling.
INTENDED FOR R & D USE ONLY.
NOT ON THE TSCA INVENTORY.
Label First Aid:
If swallowed, induce vomiting immediately as directed by medical personnel. Never give anything by mouth to an unconscious person. If inhaled, remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Call a physician. In case of contact, immediately flush eyes or skin with plenty of water for at least 15 minutes. Get medical attention if irritation develops or persists.
Product Use:
Research and Development Use Only. A DNA staining and intercalating agent for plasmid DNA isolation and gel electrophoresis.
Revision Information:
No Changes.
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Prepared by: Environmental Health & Safety
Phone Number: (314) 654-1600 (U.S.A.)