Hematoxylin (Harris) Staining Solution MSDS

Hematoxylin (Harris) Staining Solution MSDS

 

Section 1: Chemical Product and Company Identification

Product Name: Hematoxylin (Harris) Staining Solution

Catalog Codes: SLH2515

CAS#: Mixture.

RTECS: Not applicable.

TSCA: TSCA 8(b) inventory: Hematoxylin; Ethylene glycol;

Aluminum sulfate; Sodium iodate; TRITON X-114; Acetic

acid; Water

CI#: Not applicable.

Synonym: Papanicolaou Hematoxylin

Chemical Name: Not applicable.

Chemical Formula: Not applicable.

 

Section 2: Composition and Information on Ingredients

Composition:

Name CAS # % by Weight

Hematoxylin 517-28-2 0.5

Ethylene glycol 107-21-1 24

4.4

Aluminum sulfate 10043-01-3

Sodium iodate 7681-55-2 0.05

Ethylene oxide 75-21-8 0-0.00005

Acetic acid 64-19-7 1

Water 7732-18-5 70

Toxicological Data on Ingredients: Hematoxylin LD50: Not available. LC50: Not available. Ethylene glycol: ORAL (LD50):

Acute: 4700 mg/kg [Rat]. 7500 mg/kg [Mouse]. 6610 mg/kg [Guinea pig]. DERMAL (LD50): Acute: 9530 mg/kg [Rabbit]. Acetic

acid: ORAL (LD50): Acute: 3310 mg/kg [Rat]. 4960 mg/kg [Mouse]. 3530 mg/kg [Rat]. DERMAL (LD50): Acute: 1060 mg/kg

[Rat].

 

Section 3: Hazards Identification

Potential Acute Health Effects:

Very hazardous in case of ingestion. Hazardous in case of skin contact (irritant), of eye contact (irritant), of

inhalation. Slightly hazardous in case of skin contact (corrosive, permeator). Liquid or spray mist may produce

tissue damage particularly on mucous membranes of eyes, mouth and respiratory tract. Skin contact may

produce burns. Inhalation of the spray mist may produce severe irritation of respiratory tract, characterized by

coughing, choking, or shortness of breath.

Potential Chronic Health Effects:

Slightly hazardous in case of ingestion, of inhalation.

Non-corrosive for skin. Non-sensitizer for skin. Non-permeator by skin. Non-irritating to the eyes.

CARCINOGENIC EFFECTS: Not available.

MUTAGENIC EFFECTS: Not available.

TERATOGENIC EFFECTS: Not available.

DEVELOPMENTAL TOXICITY: PROVEN [Ethylene glycol]

The substance is toxic to blood, kidneys, lungs, the nervous system, the reproductive system, liver, mucous

membranes.

Repeated or prolonged exposure to the substance can produce target organs damage. Repeated or prolonged

contact with spray mist may produce chronic eye irritation and severe skin irritation. Repeated or prolonged

exposure to spray mist may produce respiratory tract irritation leading to frequent attacks of bronchial infection.

 

Section 4: First Aid Measures

Eye Contact:

Check for and remove any contact lenses. Immediately flush eyes with running water for at least 15 minutes,

keeping eyelids open. Cold water may be used. Do not use an eye ointment. Seek medical attention.

Skin Contact:

After contact with skin, wash immediately with plenty of water. Gently and thoroughly wash the contaminated skin

with running water and non-abrasive soap. Be particularly careful to clean folds, crevices, creases and groin.

Cold water may be used. Cover the irritated skin with an emollient. If irritation persists, seek medical attention.

Wash contaminated clothing before reusing.

Serious Skin Contact:

Wash with a disinfectant soap and cover the contaminated skin with an anti-bacterial cream. Seek immediate

medical attention.

Inhalation: Allow the victim to rest in a well ventilated area. Seek immediate medical attention.

Serious Inhalation:

Evacuate the victim to a safe area as soon as possible. Loosen tight clothing such as a collar, tie, belt or

waistband. If breathing is difficult, administer oxygen. If the victim is not breathing, perform mouth-to-mouth

resuscitation. WARNING: It may be hazardous to the person providing aid to give mouth-to-mouth resuscitation

when the inhaled material is toxic, infectious or corrosive. Seek immediate medical attention.

Ingestion:

Do not induce vomiting. Examine the lips and mouth to ascertain whether the tissues are damaged, a possible

indication that the toxic material was ingested; the absence of such signs, however, is not conclusive. Loosen

tight clothing such as a collar, tie, belt or waistband. If the victim is not breathing, perform mouth-to-mouth

resuscitation. Seek immediate medical attention.

Serious Ingestion: Not available.

 

Section 5: Fire and Explosion Data

Flammability of the Product: Non-flammable.

Auto-Ignition Temperature: Not applicable.

Flash Points: Not applicable.

Flammable Limits: Not applicable.

Products of Combustion: Not available.

Fire Hazards in Presence of Various Substances: Not applicable.

Explosion Hazards in Presence of Various Substances:

Risks of explosion of the product in presence of mechanical impact: Not available.

Risks of explosion of the product in presence of static discharge: Not available.

Fire Fighting Media and Instructions: Not applicable.

Special Remarks on Fire Hazards: Not available.

Special Remarks on Explosion Hazards: Not available.

 

Section 6: Accidental Release Measures

Small Spill:

Dilute with water and mop up, or absorb with an inert dry material and place in an appropriate waste disposal

container. If necessary: Neutralize the residue with a dilute solution of sodium carbonate.

Large Spill:

Corrosive liquid.

Stop leak if without risk. Absorb with DRY earth, sand or other non-combustible material. Do not get water inside

container. Do not touch spilled material. Use water spray curtain to divert vapor drift. Prevent entry into sewers,

basements or confined areas; dike if needed. Call for assistance on disposal. Neutralize the residue with a

dilute solution of sodium carbonate. Be careful that the product is not present at a concentration level above

TLV. Check TLV on the MSDS and with local authorities.

 

Section 7: Handling and Storage

Precautions:

Keep container dry. Do not ingest. Do not breathe gas/fumes/ vapour/spray. Never add water to this product In

case of insufficient ventilation, wear suitable respiratory equipment If ingested, seek medical advice immediately

and show the container or the label. Avoid contact with skin and eyes

Storage: Corrosive materials should be stored in a separate safety storage cabinet or room.

 

Section 8: Exposure Controls/Personal Protection

Engineering Controls:

Provide exhaust ventilation or other engineering controls to keep the airborne concentrations of vapors below their

respective threshold limit value. Ensure that eyewash stations and safety showers are proximal to the

work-station location.

Personal Protection:

Splash goggles. Lab coat. Vapor respirator. Be sure to use an approved/certified respirator or equivalent.

Gloves.

Personal Protection in Case of a Large Spill:

Splash goggles. Full suit. Vapor respirator. Boots. Gloves. A self contained breathing apparatus should be

used to avoid inhalation of the product. Suggested protective clothing might not be sufficient; consult a specialist

BEFORE handling this product.

Exposure Limits:

Ethylene glycol

CEIL: 50 (ppm) from ACGIH (TLV)

p. 3

CEIL: 125 (mg/m3) from ACGIH

Aluminum sulfate

TWA: 0.14 (ppm)

Acetic acid

TWA: 10 CEIL: 15 (ppm)

TWA: 25 CEIL: 37 (mg/m3)

Consult local authorities for acceptable exposure limits.

 

Section 9: Physical and Chemical Properties

Physical state and appearance: Liquid.

Odor: Not available.

Taste: Not available.

Molecular Weight: Not applicable.

Color: Not available.

pH (1% soln/water): Acidic.

Boiling Point: The lowest known value is 100C (212F) (Water). Weighted average: 124.85C (256.7F)

Melting Point: May start to solidify at 0C (32F) based on data for: Acetic acid. Weighted average: -12.48C (9.5F)

Critical Temperature: Not available.

Specific Gravity: Weighted average: 1.06 (Water = 1)

Vapor Pressure: The highest known value is 17.535 mm of Hg (@ 20C) (Water). Weighted average: 13.05 mm of Hg (@

20C)

Vapor Density: The highest known value is 2.14 (Air = 1) (Ethylene glycol). Weighted average: 1.02 (Air = 1)

Volatility: Not available.

Odor Threshold: The highest known value is 1.018 ppm (Acetic acid)

Water/Oil Dist. Coeff.: The product is equally soluble in oil and water.

Ionicity (in Water): Not available.

Dispersion Properties: See solubility in water, methanol, diethyl ether, n-octanol, acetone.

Solubility: Easily soluble in cold water, hot water, methanol, diethyl ether, n-octanol, acetone.

 

Section 10: Stability and Reactivity Data

Stability: The product is stable.

Instability Temperature: Not available.

Conditions of Instability: Not available.

Incompatibility with various substances: Not available.

Corrosivity: Non-corrosive in presence of glass.

Special Remarks on Reactivity: Sensitive to light. (Hematoxylin)

Special Remarks on Corrosivity: Not available.

Polymerization: No.

 

Section 11: Toxicological Information

Routes of Entry: Eye contact. Inhalation. Ingestion.

Toxicity to Animals:

Acute oral toxicity (LD50): 3310 mg/kg [Rat]. (Acetic acid).

Acute dermal toxicity (LD50): 1060 mg/kg [Rat]. (Acetic acid).

Chronic Effects on Humans:

DEVELOPMENTAL TOXICITY: PROVEN [Ethylene glycol]

The substance is toxic to blood, kidneys, lungs, the nervous system, the reproductive system, liver, mucous

membranes.

Other Toxic Effects on Humans:

Very hazardous in case of ingestion.

Hazardous in case of skin contact (irritant), of inhalation.

Slightly hazardous in case of skin contact (corrosive, permeator).

Special Remarks on Toxicity to Animals: Not available.

Special Remarks on Chronic Effects on Humans: Passes through the placental barrier in animal. (Ethylene glycol)

Special Remarks on other Toxic Effects on Humans: Not available.

 

Section 12: Ecological Information

Ecotoxicity: Not available.

BOD5 and COD: Not available.

Products of Biodegradation:

Possibly hazardous short term degradation products are not likely. However, long term degradation products may

arise.

Toxicity of the Products of Biodegradation: The products of degradation are more toxic.

Special Remarks on the Products of Biodegradation: Not available.

 

Section 13: Disposal Considerations

Waste Disposal:

 

Section 14: Transport Information

DOT Classification: CLASS 8: Corrosive liquid.

Identification: : Corrosive liquids n.o.s. (Acetic acid) : UN1760 PG: II

Special Provisions for Transport: Not available.

 

Section 15: Other Regulatory Information

Federal and State Regulations:

California prop. 65: This product contains the following ingredients for which the State of California has found to

cause cancer, birth defects or other reproductive harm, which would require a warning under the statute: Ethylene

oxide

California prop. 65: This product contains the following ingredients for which the State of California has found to

cause reproductive harm (male) which would require a warning under the statute: Ethylene oxide

California prop. 65: This product contains the following ingredients for which the State of California has found to

cause birth defects which would require a warning under the statute: Ethylene oxide

California prop. 65: This product contains the following ingredients for which the State of California has found to

cause cancer which would require a warning under the statute: Ethylene oxide

Pennsylvania RTK: Ethylene glycol; Aluminum sulfate; Ethylene oxide; Acetic acid

Florida: Ethylene oxide

Minnesota: Ethylene oxide

Massachusetts RTK: Ethylene glycol; Aluminum sulfate; Ethylene oxide; Acetic acid

New Jersey: Ethylene oxide

TSCA 8(b) inventory: Hematoxylin; Ethylene glycol; Aluminum sulfate; Sodium iodate; TRITON X-114; Acetic

acid; Water

SARA 302/304/311/312 extremely hazardous substances: Ethylene oxide

SARA 313 toxic chemical notification and release reporting: Ethylene oxide

CERCLA: Hazardous substances.: Ethylene glycol; Aluminum sulfate; Acetic acid;

Other Regulations: OSHA: Hazardous by definition of Hazard Communication Standard (29 CFR 1910.1200).

Other Classifications:

WHMIS (Canada):

CLASS D-2A: Material causing other toxic effects (VERY TOXIC).

CLASS E: Corrosive liquid.

DSCL (EEC): R34- Causes burns.

HMIS (U.S.A.):

Health Hazard: 1

Fire Hazard: 0

Reactivity: 0

Personal Protection: h

National Fire Protection Association (U.S.A.):

Health: 1

Flammability: 1

Reactivity: 0

Specific hazard:

Protective Equipment:

Gloves.

Lab coat.

Vapor respirator. Be sure to use an

approved/certified respirator or

equivalent. Wear appropriate respirator

when ventilation is inadequate.

Splash goggles.

 

Section 16: Other Information

References: Not available.

Other Special Considerations: Not available.

Created: 10/10/2005 10:25 AM

Last Updated: 11/06/2008 12:00 PM