Toluidine Blue For Microscopy MSDS
Section 1: Chemical Product and Company Identification
Product Name: Toluidine Blue For Microscopy
Catalog
Codes: SLT3952
CAS#:
6568-04-5
RTECS:
SP5670000
TSCA: TSCA 8(b) inventory: Toluidine Blue For Microscopy
CI#: 52040
Synonym: Basic Blue 17; Tolonium chloride; Blutene
chloride; C.I. Basic Blue 17; Gabilin; Menodin
Chemical Name: 3-Amino-7-(dimethylamino)-2-
methylphenothiazin-5-ium chloride;
Chemical
Formula: C15-H16-N3-S-Cl
Section 2: Composition and Information on Ingredients
Composition:
Name CAS # % by Weight
Toluidine Blue For Microscopy 6568-04-5 100
Toxicological Data on Ingredients: Not applicable.
Section 3: Hazards Identification
Potential Acute Health Effects: Slightly hazardous in case of skin contact (irritant), of eye contact (irritant), of ingestion, of
inhalation.
Potential Chronic Health Effects:
CARCINOGENIC EFFECTS: Not available. MUTAGENIC EFFECTS: Mutagenic for bacteria and/or yeast. TERATOGENIC
EFFECTS: Not available. DEVELOPMENTAL TOXICITY: Not available. Repeated or prolonged exposure is not known to
aggravate medical condition.
Section 4: First Aid Measures
Eye Contact:
Check for and remove any contact lenses. In case of contact, immediately flush eyes with plenty of water for at least 15
minutes. Cold water may be used. Get medical attention if irritation occurs.
Skin Contact:
Wash with soap and water. Cover the irritated skin with an emollient. Get medical attention if irritation develops. Cold water
may be used.
Serious Skin Contact: Not available.
Inhalation:
If inhaled, remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical
attention.
Serious Inhalation: Not available.
Ingestion:
Do NOT induce vomiting unless directed to do so by medical personnel. Never give anything by mouth to an unconscious
person. If large quantities of this material are swallowed, call a physician immediately. Loosen tight clothing such as a collar,
tie, belt or waistband.
Serious Ingestion: Not available.
Section 5: Fire and Explosion Data
Flammability of the Product: May be combustible at high temperature.
Auto-Ignition Temperature: Not available.
Flash Points: Not available.
Flammable Limits: Not available.
Products of Combustion: These products are carbon oxides (CO, CO2), nitrogen oxides (NO, NO2...), sulfur oxides (SO2,
SO3...).
Fire Hazards in Presence of Various Substances: Slightly flammable to flammable in presence of heat.
Explosion Hazards in Presence of Various Substances:
Risks of explosion of the product in presence of mechanical impact: Not available. Risks of explosion of the product in
presence of static discharge: Not available.
Fire Fighting Media and Instructions:
SMALL FIRE: Use DRY chemical powder. LARGE FIRE: Use water spray, fog or foam. Do not use water jet.
Special Remarks on Fire Hazards: As with most organic solids, fire is possible at elevated temperatures
Special Remarks on Explosion Hazards: Not available.
Section 6: Accidental Release Measures
Small Spill:
Use appropriate tools to put the spilled solid in a convenient waste disposal container. Finish cleaning by spreading water on
the contaminated surface and dispose of according to local and regional authority requirements.
Large Spill:
Use a shovel to put the material into a convenient waste disposal container. Finish cleaning by spreading water on the
contaminated surface and allow to evacuate through the sanitary system.
Section 7: Handling and Storage
Precautions:
Keep away from heat. Keep away from sources of ignition. Ground all equipment containing material. Do not breathe dust.
Wear suitable protective clothing. If you feel unwell, seek medical attention and show the label when possible. Keep away from
incompatibles such as oxidizing agents.
Storage: Keep container tightly closed. Keep container in a cool, well-ventilated area.
Section 8: Exposure Controls/Personal Protection
Engineering Controls:
Use process enclosures, local exhaust ventilation, or other engineering controls to keep airborne levels below recommended
exposure limits. If user operations generate dust, fume or mist, use ventilation to keep exposure to airborne contaminants
below the exposure limit.
Personal Protection: Safety glasses. Lab coat. Dust respirator. Be sure to use an approved/certified respirator or equivalent.
Gloves.
Personal Protection in Case of a Large Spill:
Splash goggles. Full suit. Dust respirator. Boots. Gloves. A self contained breathing apparatus should be used to avoid
inhalation of the product. Suggested protective clothing might not be sufficient; consult a specialist BEFORE handling this
product.
Exposure Limits: Not available.
Section 9: Physical and Chemical Properties
Physical state and appearance: Solid. (Powder.)
Odor: Not available.
Taste: Not available.
Molecular Weight: 305.83 g/mole
Color: Green. (Dark.)
pH (1% soln/water): Not available.
Boiling Point: Not available.
Melting Point: Not available.
Critical Temperature: Not available.
Specific Gravity: Not available.
Vapor Pressure: Not applicable.
Vapor Density: Not available.
Volatility: Not available.
Odor Threshold: Not available.
Water/Oil Dist. Coeff.: Not available.
Ionicity (in Water): Not available.
Dispersion Properties: See solubility in water.
Solubility:
Soluble in methanol. Partially soluble in cold water. Solubility in Water: 3.82 g/100 ml
Section 10: Stability and Reactivity Data
Stability: The product is stable.
Instability Temperature: Not available.
Conditions of Instability: Excess heat, incompatible materials
Incompatibility with various substances: Reactive with oxidizing agents.
Corrosivity: Non-corrosive in presence of glass.
Special Remarks on Reactivity: Not available.
Special Remarks on Corrosivity: Not available.
Polymerization: Will not occur.
Section 11: Toxicological Information
Routes of Entry: Inhalation. Ingestion.
Toxicity to Animals:
LD50: Not available. LC50: Not available.
Chronic Effects on Humans: MUTAGENIC EFFECTS: Mutagenic for bacteria and/or yeast.
Other Toxic Effects on Humans: Slightly hazardous in case of skin contact (irritant), of ingestion, of inhalation.
Special Remarks on Toxicity to Animals: Not available.
Special Remarks on Chronic Effects on Humans: May affect genetic material (mutagenic)
Special Remarks on other Toxic Effects on Humans:
Acute Potential Health Effects: Skin: May cause skin irritation. Eyes: May cause eye irritation. Inhalation: May cause
respiratory tract irritation. Ingestion: May cause nausea, vomiting, abdominal pain, diarrhea, dyspnea (difficulty breathing),
bluish tint of the skin. May cause burning on urination, and the urine to become dark blue to green depending on the
concentration in the urine. There have been reports on effects of Toluidine Blue For Microscopy on the blood (hemolytic anemia, leukopenia,
methemoglobinemia), on behavior/central nervous system (headache, convulsions), cardiovascular system (hypertension,
dysrthymia) as well as reports of acute renal failure and hematuria (only after hemolysis). However, these effects occured only
after intravenous dosage.
Section 12: Ecological Information
Ecotoxicity: Not available.
BOD5 and COD: Not available.
Products of Biodegradation:
Possibly hazardous short term degradation products are not likely. However, long term degradation products may arise.
Toxicity of the Products of Biodegradation: The product itself and its products of degradation are not toxic.
Special Remarks on the Products of Biodegradation: Not available.
Section 13: Disposal Considerations
Waste Disposal:
Waste must be disposed of in accordance with federal, state and local environmental control regulations.
Section 14: Transport Information
DOT Classification: Not a DOT controlled
material (
Identification: Not applicable.
Special Provisions for Transport: Not applicable.
Section 15: Other Regulatory Information
Federal and State Regulations: TSCA 8(b) inventory: Toluidine Blue For Microscopy
Other Regulations: EINECS: This product is on the European Inventory of Existing Commercial Chemical Substances.
Other Classifications:
WHMIS (
DSCL (EEC):
This product is not classified according to the EU regulations. Not applicable.
HMIS (
Health Hazard: 1
Fire Hazard: 1
Reactivity: 0
Personal Protection: E
National Fire Protection Association (
Health: 1
Flammability: 1
Reactivity: 0
Specific hazard:
Protective Equipment:
Gloves. Lab coat. Dust respirator. Be sure to use an approved/certified respirator or equivalent. Safety glasses.
Section 16: Other Information
References: Not available.
Other Special Considerations: Not available.
Created: 10/11/2005 12:47 PM
Last Updated: 11/06/2008 12:00 PM